Engineering Building, Room E 104

Agenda: 6:30PM - Pizza and Pop

                7:00PM - Presentation followed by Q&A

                8:00PM - Conclude

                Free and Open to All.
 
Presentation:
solid-state nanopores as single molecule sensors

Diego Krapf, Electrical and Computer Engineering, Colorado State University

ABSTRACT
 

Nanopores are single nanometer-sized holes in thin insulating membranes, which present a new tool for the detection and analysis of individual biopolymers such as DNA.  Applying a voltage bias, negatively charged DNA molecules are pulled through the pore by the electric field.  When a molecule enters the channel, it displaces its own volume of solution and hence it alters the pore ionic conductance.  By recording the ionic current it is possible to detect the passage of individual molecules.

We have started an effort to combine solid-state nanopores with optical tweezers to study real-time dynamics of DNA and protein-DNA interactions.  Individual DNA molecules can be electrically driven into a nanopore while holding one end of the DNA with the optical tweezers.  As a result, the DNA translocation is stalled at a specific site and its position is controlled with nanometer accuracy.  The capture of DNA inside the pore is detected as changes in both the ionic conductance and the bead position (force detection).  We have demonstrated the capabilities of this technique by locally measuring the electric force exerted on a DNA molecule during translocation through a 10-nm pore.  These measurements allow us to gain insight into the microscopic structure of the ionic atmosphere surrounding DNA polymers.  We are currently using nanopores in combination with optical tweezers to understand the polymerization kinetics of DNA binding proteins.

Presenter Bio

Dr. Diego Krapf received his M.Sc. and Ph.D. in Applied Physics from the School of Applied Science at the Hebrew University of Jerusalem.  His research interests include single-molecule biophysics; Nanofluidic devices; Protein-DNA interactions; and Chromatin Dynamics.